normal mouse fibroblast cells l929  (Procell Inc)

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Royal jelly induces ROS-mediated apoptosis in acute lymphoblastic leukemia (ALL)-derived Nalm-6 cells: Shedding light on novel therapeutic approaches for ALL

doi: 10.22038/IJBMS.2024.76261.16498

Figure Lengend Snippet: Effect of royal jelly (RJ) on normal cells. L929 and PBMC were used as models of normal cells. The MTT assay indicated that RJ has no cytotoxic effect on L929 and PBMC cells (A). Analysis of apoptosis using Annexin-V/PI staining and flow cytometry revealed that RJ has no significant apoptotic effect on normal cells (B). Hemolytic activity evolution suggested that the hemolysis rate decreases when RJ concentration increases (C). The values are given as the mean SD of three independent experiments. * P ≤0.05 represents significant statistical changes from the control sample

Article Snippet: Cell culture and treatment Acute Lymphoblastic Leukemia cell line (Nalm-6) and normal mouse fibroblast cell line (L929) were purchased from the Pasteur Institute collection (Tehran, Iran).

Techniques: MTT Assay, Staining, Flow Cytometry, Activity Assay, Concentration Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Comparison Study of Cytotoxicity of Bare and Functionalized Zinc Oxide Nanoparticles

doi: 10.3390/ijms22179529

Figure Lengend Snippet: The MTT assay results and effects of indicated concentrations of bare and functionalized ZnO NPs on the ( A ) Caco-2 cells and ( B ) L929 cell viability. ( C ) the effect of different concentrations of biochemically synthetized ZnO NPs against the Caco-2 cell line. The values are expressed as mean ± SD values of three independent experiments ( n = 3); * p < 0.001 compared to bare ZnO NPs (ANOVA).

Article Snippet: The L929 normal mouse fibroblast cells and Caco-2 from human colon were obtained from the European Collection of Authenticated Cell Cultures.

Techniques: MTT Assay

Journal: International Journal of Molecular Sciences

Article Title: Comparison Study of Cytotoxicity of Bare and Functionalized Zinc Oxide Nanoparticles

doi: 10.3390/ijms22179529

Figure Lengend Snippet: LDH leakage level of Caco-2 and L929 cells treated with different concentrations of ( A ) chemically, ( B ) biochemically and ( C ) intracellularly synthetized ZnO NPs. The values are expressed as mean ± SD values of three independent experiments ( n = 3); * p < 0.001 (ANOVA).

Article Snippet: The L929 normal mouse fibroblast cells and Caco-2 from human colon were obtained from the European Collection of Authenticated Cell Cultures.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Comparison Study of Cytotoxicity of Bare and Functionalized Zinc Oxide Nanoparticles

doi: 10.3390/ijms22179529

Figure Lengend Snippet: SEM analysis showing the morphology changes in L929 cells after the bare and functionalized ZnO NPs treatement. ( A ) control sample and L929 cells after the ( B ) extracellularly, ( C ) phytochemically, ( D ) intracellularly, ( E ) chemically and ( F ) biochemically synthetized ZnO NPs’ exposure. The concentration of the used ZnO NPs was 6.25 μg/mL.

Article Snippet: The L929 normal mouse fibroblast cells and Caco-2 from human colon were obtained from the European Collection of Authenticated Cell Cultures.

Techniques: Control, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Comparison Study of Cytotoxicity of Bare and Functionalized Zinc Oxide Nanoparticles

doi: 10.3390/ijms22179529

Figure Lengend Snippet: Concentration-dependent ROS generation by bare and functionalized ZnO-NPs in ( A ) L929 and ( B ) Caco-2 cells. The values are expressed as mean ± SD values of three independent experiments ( n = 3); * p < 0.001 compared to bare ZnO NPs (ANOVA).

Article Snippet: The L929 normal mouse fibroblast cells and Caco-2 from human colon were obtained from the European Collection of Authenticated Cell Cultures.

Techniques: Concentration Assay